The final magnification of the details of specimen under observation is the product of both the eye-piece, and objective lenses (Caprette, 2005).For one to be able to note the details of the specimen, staining is necessary. Different substances will absorb light differently, thus transmit light in different proportions. The difference in light transmission through the different parts due to staining makes the isolation of details possible. The extent to which a microscope differentiates fine details in the specimen is called the resolution. In the light microscope, the resolution can be obtained as below:The resolution becomes smaller as the resolution of the microscope improves. The value ‘n’ is increased by using a drop of oil. The drop is placed between the objective lens and the specimen. With this type of microscope, the resolution ranges between 0.2µm and 200nm. This is because the λ of light is 450nm (Karp, 2009, p. 718).The intention of this part of the practical is to obtain cells from the cheek, and observe the cell structure under a light microscope. The staining was done using eosin, and haematoxylin dyes. This procedure was done while wearing a glove since it involved coming into contact with human fluid, specifically saliva. Wearing gloves was a safety measure that is a procedural requirement when dealing with specimen from animals.5. The contents of the tube were poured into a Petri dish. The root tips were carefully picked out using a forceps and placed in an Eppendorf tube which contained aceto-orcein. The mixture was left in the dark for 10 minutes.7. The softened root tip, which by now had softened, was squashed. It was then stained by lightly tapping lightly on the coverslip using a pencil. On doing this the root tip spread out as a pink mass.Enzymes are proteins that are distributed in all cellular compartments. In definition, enzymes are proteins which catalyze biological processes.
Biology Practical on Cells